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Petri to Liquid Culture Jar Transfer Procedure

For sterile transfer of mycelium from petri dish to quart jar for expanding liquid culture

Required Equipment

Essential Tools

  • Bio safety cabinet/flow hood
  • Petri stand
  • Petri suction cup
  • Scalpel
  • Alcohol tray
  • Bacti-Incinerator
  • Parafilm
  • Labels

Safety & Sanitation

  • 70% Isopropyl alcohol
  • Sterile arm sleeves
  • Nitrile gloves
  • Alcohol
  • Towelettes

Cultures And Prepared Media

  • Source petri dishes
  • Sterilized liquid culture jars (Pre-prepared with nutrient broth)

Pre-Transfer Procedure

Initial Setup

  • Turn on and preheat 
  • Bacti-Incinerator
  • Wipe base with alcohol
  • Position in hood

Bio Cabinet Preparation

  • Don gloves and arm sleeves
  • Sanitize hood interior and arm ports
  • Lower hood sash
  • Arrange workspace left-to-right

Equipment Sterilization

  • Sanitize all equipment with alcohol
  • Carefully clean petri dishes (avoid label smearing)
  • Place materials in hood following directional workflow

Transfer Procedure

Workspace Setup

  • Adjust chair height for optimal reach
  • Sanitize gloves thoroughly
  • Remove parafilm from source petri
  • Position dish face-down on stand
  • Pre-loosen jar lids under laminar flow

Sample Collection

  • Attach suction cup to working petri
  • Sterilize scalpel (25 seconds in Bacti-Incinerator)
  • Cool scalpel in air flow
  • Lift petri with suction cup facing upward
  • Cut triangular sample from outer colony edge
  • Collect sample on scalpel tip

Jar Inoculation

  • Return petri to stand
  • Remove jar lid maintaining sterile technique
  • Transfer sample into broth
  • Tap scalpel gently to release sample
  • Reseal jar ensuring proper gasket alignment

Post-Transfer Protocol

Finishing Steps 

  • Reseal petri dish with fresh parafilm
  • Label jars with: Species
  • Date Source plate info
  • Place jars in incubation room
  • Return source plates to storage

Quality Control Notes

  • Maintain sterile technique throughout
  • Work within laminar flow Avoid crossing over sterile field
  • Monitor for contamination signs
  • Document all transfers

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