Grain spawn is expanded culture of a specific species used to inoculate larger blocks and further expand it's volume. It is often used as an intermediary step to larger fruiting blocks. The reason is because the transfer procedure is fast to perform and the mycelium expands much quicker in grain than is does on it's final fruiting substrate. This results in faster incubation times, and ultimately faster fruiting times. In this article, we will provide you with step-by-step instructions to ensure successful transfer and inoculation.
Before we begin, it's important to note that Liquid Fungi also provides 5 lb. high-quality pre-sterilized mushroom fruiting blocks that can be used immediately. This allows you to skip the substrate prep and sterilization below.
Step 1: Prepare Your Work Area
Creating a sterile work area is crucial to prevent contamination during the inoculation process. Disinfect everything with 70% isopropyl alcohol. Make a clean and sanitized workspace. Preferably work in a laminar flow hood or a still air box. If you do not have one, be sure to minimize exposure to open air. Turn off all fans and AC in your place to make the room still.
Step 2: Disinfect the Bags
Wipe the surface of both the source grain spawn bag, and the target substrate bag with alcohol to sterilize it.
Step 3: Transfer Grain Spawn
Cut the top off the Grain Spawn bag and the target substrate bag. Open the Large Mushroom Grow Bag and gently add the grain spawn to the substrate. Be careful not to bring hands directly above the bag.
Step 4: Seal and Incubate
Seal the Large Mushroom Grow Bag securely and place it in a suitable incubation environment. Maintain the recommended temperature for your mushroom species. Allow the mycelium to colonize the substrate fully.